Prostaglandin biosynthesis and lipolysis in subcellular fractions from rabbit kidney medulla.

Effects of divalent cations on prostaglandin biosynthesis and phospholipase A2 activation in rabbit kidney medulla slices.

The divalent cations Ca2+, Mg2+, Co2+, Mn2+, Sr2+, and Ba2+ were compared for their stimulatory or inhibitory effect on PG formation in rabbit kidney medulla slices. Calcium, manganese, and strontium ions stimulated PG generation up to 3- to 5-fold in a time- and dose-dependent manner (Ca2+ greater than Mn2+ approximately Sr2+) while the magnesium and cobalt ions were without significant effect...

Exaggerated prostaglandin biosynthesis and its influence on renal resistance in the isolated hydronephrotic rabbit kidney.

Basal and hormone-stimulated prostaglandin biosynthesis was compared in isolated perfused rabbit kidneys with and without ureteral obstruction. At 72 h there was enhanced responsiveness to bradykinin in the ureter-obstructed hydronephrotic kidney. The amount of prostaglandin-like substance released from the perfused kidneys by 25 ng of bradykinin was 533+/-163 ng from the ureter-obstructed, 28+...

Microsomal Prostaglandin Biosynthesis

We have investigated prostaglandin biosynthesis by human renal medullary and cortical microsomes with arachidonic acid as substrate. Prostaglandins were measured by two independent methods, namely radiometric thin layer chromatography and radioimmunoassay. The relative abundance of various prostaglandins synthesized by microsomes was similar in cortex and medulla. In the absence of added cofact...

Effect of diarachidonin on prostaglandin E2 synthesis in rabbit kidney medulla slices.

The effect of diarachidonin on the synthesis of prostaglandin E2 in rabbit kidney medulla slices was examined. The addition of diarachidonin stimulated prostaglandin E2 production in a dose-dependent manner. At three concentrations (10, 50 and 100 microM), increases in prostaglandin E2 formation induced by exogenous diarachidonin were 2-fold greater than those induced by exogenous arachidonic a...

Inhibition of prostaglandin synthetase system in the rabbit renal medulla by psychotropic drugs.